Introduction to Open Data Science - Course Project

About the project

Write a short description about the course and add a link to your GitHub repository here. This is an R Markdown (.Rmd) file so you should use R Markdown syntax.

# This is a so-called "R chunk" where you can write R code.

date()
## [1] "Sat Dec 05 20:14:01 2020"

The text continues here.

Learning R is an interesting process even though there are a lot of difficulties which can not be sloved sometimes. I hope I could learn more and more in this course.I found this course in weboodi.

Regression and model validation

This week I learned how to use R to wrangling data and make the linear regression analysis and evaluate the validation of model.

Here we go again…

In the exercise 2, the Code is as following:

This data files includes 166 rows (observations) and 7 columns(variables), which means there are 7 kinds of index for 166 students.The variables includes gender, age, attuitude, deep, dtra, surf, and points.

students2014 <- read.table('http://s3.amazonaws.com/assets.datacamp.com/production/course_2218/datasets/learning2014.txt', sep = ',', header = TRUE)
dim(students2014)
## [1] 166   7
str(students2014)
## 'data.frame':    166 obs. of  7 variables:
##  $ gender  : chr  "F" "M" "F" "M" ...
##  $ age     : int  53 55 49 53 49 38 50 37 37 42 ...
##  $ attitude: num  3.7 3.1 2.5 3.5 3.7 3.8 3.5 2.9 3.8 2.1 ...
##  $ deep    : num  3.58 2.92 3.5 3.5 3.67 ...
##  $ stra    : num  3.38 2.75 3.62 3.12 3.62 ...
##  $ surf    : num  2.58 3.17 2.25 2.25 2.83 ...
##  $ points  : int  25 12 24 10 22 21 21 31 24 26 ...
library(ggplot2)
## Warning: package 'ggplot2' was built under R version 4.0.2
p1 <- ggplot(students2014, aes(x=attitude, y=points, col=gender))
p2 <- p1 + geom_point()
p2

p3 <- p2+geom_smooth(method = 'lm')
p4 <- p3+ggtitle('attitude of students versus exam points')
p4
## `geom_smooth()` using formula 'y ~ x'

pairs(students2014[-1])

library(GGally)
## Warning: package 'GGally' was built under R version 4.0.3
## Registered S3 method overwritten by 'GGally':
##   method from   
##   +.gg   ggplot2
library(ggplot2)

p <- ggpairs(students2014, mapping=aes(col=gender, alpha=0.3),lowe=list(combo=wrap('facethist', bins=20)))
p

There is an extremely significant positive relationship between attitude of students and points.For male,age is negative related with points.

my_model1 <- lm (points ~ attitude + stra + surf, data = students2014)
summary(my_model1)
## 
## Call:
## lm(formula = points ~ attitude + stra + surf, data = students2014)
## 
## Residuals:
##      Min       1Q   Median       3Q      Max 
## -17.1550  -3.4346   0.5156   3.6401  10.8952 
## 
## Coefficients:
##             Estimate Std. Error t value Pr(>|t|)    
## (Intercept)  11.0171     3.6837   2.991  0.00322 ** 
## attitude      3.3952     0.5741   5.913 1.93e-08 ***
## stra          0.8531     0.5416   1.575  0.11716    
## surf         -0.5861     0.8014  -0.731  0.46563    
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
## 
## Residual standard error: 5.296 on 162 degrees of freedom
## Multiple R-squared:  0.2074, Adjusted R-squared:  0.1927 
## F-statistic: 14.13 on 3 and 162 DF,  p-value: 3.156e-08
my_model2 <- lm (points ~ attitude + stra, data = students2014)
summary(my_model2)
## 
## Call:
## lm(formula = points ~ attitude + stra, data = students2014)
## 
## Residuals:
##      Min       1Q   Median       3Q      Max 
## -17.6436  -3.3113   0.5575   3.7928  10.9295 
## 
## Coefficients:
##             Estimate Std. Error t value Pr(>|t|)    
## (Intercept)   8.9729     2.3959   3.745  0.00025 ***
## attitude      3.4658     0.5652   6.132 6.31e-09 ***
## stra          0.9137     0.5345   1.709  0.08927 .  
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
## 
## Residual standard error: 5.289 on 163 degrees of freedom
## Multiple R-squared:  0.2048, Adjusted R-squared:  0.1951 
## F-statistic: 20.99 on 2 and 163 DF,  p-value: 7.734e-09

In the first regression model, points as the target varable together with attitude, stra and surf as explantory variables, the model can be used because the P value is less than 0.001(extremely significant) even though there is no significantly relationships between stra, surf and points.

Model2 can explain the 20.74% changes of points.

par(mfrow=c(2,2))
plot(my_model2, which=c(1,2,5))

The data is mostly Gaussian distribution, according to the linear qq plots. The model is sensible based on these three plots.

Logistic Regression and cross validation

alc <- read.table('http://s3.amazonaws.com/assets.datacamp.com/production/course_2218/datasets/alc.txt', sep=',', header = TRUE)

In this data set, there are 35 variables and 382 observations in total.The variables are: school,sex,age,address,famsize,Pstatus,Medu,Fedu,Mjob,Fjob reason,nursery,internet,guardian,traveltime studytime,failures,schoolsup,famsup,paid activities,higher,romantic,famrel,freetime goout,Dalc,Walc,health,absences,G1,G2,G3,alc_use,high_use.

I chose failures, absences,traveltime and activities as explainary variables to explore the relationsips between them and the target variable-high_use.The results are as below(three of them have significatly positive relationship with high_use): high_use=0.4176failures-1.0157 high_use=0.0683absences-1.2640 high_use=0.4290*traveltime-1.5146

So, the high_use valus will increase with the increase of failures, absences and traveltime. However, there is no significant relationship between activities and high_use.

library(ggplot2)
library(tidyr)
## Warning: package 'tidyr' was built under R version 4.0.2
library(dplyr)
## Warning: package 'dplyr' was built under R version 4.0.2
## 
## Attaching package: 'dplyr'
## The following objects are masked from 'package:stats':
## 
##     filter, lag
## The following objects are masked from 'package:base':
## 
##     intersect, setdiff, setequal, union
gather(alc) %>% glimpse
## Rows: 13,370
## Columns: 2
## $ key   <chr> "school", "school", "school", "school", "school", "school", "...
## $ value <chr> "GP", "GP", "GP", "GP", "GP", "GP", "GP", "GP", "GP", "GP", "...
gather(alc) %>% ggplot(aes(value)) + facet_wrap("key", scales = "free") + geom_bar()

g1 <- ggplot(alc, aes(x = high_use, y = failures, col = sex))
g1 + geom_boxplot() + ylab("failures")

g2 <- ggplot(alc, aes(x = high_use, y = absences, col = sex))
g2 + geom_boxplot() + ggtitle("Student absences by alcohol consumption and sex")

If we look at the combined bar chart, we can find the numbers of students who attend extra-curricular activities and the students who don’t are almost close, which can tell us the non-significat relationship between activities and high_use is sensible.Let’s use the same principle to explain the other relationships, and the results should be consistent with the original hypothese.

Based on the results of Logistic regression analysis between failures&absences&traveltime&activities and high_use, the fitted model is as below:

(Intercept) failures absences traveltime activitiesyes -1.86181552 0.36618544 0.07004812 0.42610620 -0.32180313

OR 2.5 % 97.5 % (Intercept) 0.1553903 0.0828279 0.2841262 failures 1.4422227 1.0755782 1.9361180 absences 1.0725598 1.0374448 1.1130908 traveltime 1.5312834 1.1164457 2.1057338 activitiesyes 0.7248409 0.4542950 1.1528149

high_use= 0.36619 * failures+0.07005 * absences + 0.42611 * travelime -1.86182

There is no significat relationship between activities and high_use, which is same with the previous hypothese, and should be removed fromt the whole model.

The 2x2 cross tabulation of predictions is as below:

prediction

high_use FALSE TRUE

FALSE 261 9

TRUE 87 25

m5 <- glm(high_use ~ failures+absences+traveltime, data = alc, family = "binomial")
probabilities <- predict(m5, type = "response")
alc <- mutate(alc, probability = probabilities)
alc <- mutate(alc, prediction = probability > 0.5)
select(alc, failures, absences, traveltime, high_use, probability, prediction) %>% tail(10)
##     failures absences traveltime high_use probability prediction
## 373        1        0          2    FALSE   0.3105560      FALSE
## 374        1       14          2     TRUE   0.5424959       TRUE
## 375        0        2          2    FALSE   0.2616419      FALSE
## 376        0        7          3    FALSE   0.4322838      FALSE
## 377        1        0          1    FALSE   0.2285089      FALSE
## 378        0        0          1    FALSE   0.1686879      FALSE
## 379        1        0          2    FALSE   0.3105560      FALSE
## 380        1        0          2    FALSE   0.3105560      FALSE
## 381        0        3          2     TRUE   0.2752164      FALSE
## 382        0        0          3     TRUE   0.3194072      FALSE
table(high_use = alc$high_use, prediction = alc$prediction)
##         prediction
## high_use FALSE TRUE
##    FALSE   260   10
##    TRUE     88   24
g <- ggplot(alc, aes(x = probability, y = high_use, col = prediction))
g + geom_point()

table(high_use = alc$high_use, prediction = alc$prediction) %>% prop.table %>% addmargins
##         prediction
## high_use      FALSE       TRUE        Sum
##    FALSE 0.68062827 0.02617801 0.70680628
##    TRUE  0.23036649 0.06282723 0.29319372
##    Sum   0.91099476 0.08900524 1.00000000

According to the 10-folds cross-validation on your mode, I got a smaller error (0.2591623) than Datacamp, which means that my model has better test set performance.

I made two different fited model with more predictors;model6 has 5 predictors which are failures,absences,traveltime,higher as well as sex; while model 7 has 4 explainary factors.

The 10 folds cross validation results are as below:

m6-> 0.2408377 m7-> 0.2382199

Compared with m5 (0.2591623), the errors of m7 is the smallest one which has one more predictor than m5.The fitted model is:

(Intercept) failures absences traveltime sexM -2.51975566 0.36718070 0.07462037 0.39558320 0.96465837

high_use= 0.36718070 * failures + 0.07462037 * absences + 0.39558320 * traveltime + 0.96465837 * sexM -2.51975566

Clustering and classification

The boston data frame has 506 rows and 14 columns, which means that there are 14 variables. crim is per capita crime rate by town; zn is proportion of residential land zoned for lots over 25,000 sq.ft.; indus is the proportion of non-retail business acres per town; chas is Charles River dummy variable (= 1 if tract bounds river; 0 otherwise); nox is nitrogen oxides concentration (parts per 10 million); rm is average number of rooms per dwelling; age is proportion of owner-occupied units built prior to 1940; dis is weighted mean of distances to five Boston employment centres;rad is index of accessibility to radial highways; tax is full-value property-tax rate per $10,000; ptratio is pupil-teacher ratio by town.black is 1000(Bk - 0.63)^2 where Bk is the proportion of blacks by town; lstat means lower status of the population (percent); medv means median value of owner-occupied homes in $1000s.

According to the correlation matrix, there is an obverse positive relationship between rad and tax, indus and nox, while there is an negative relationship between dis and nox, indus as well as dis. Also, seems like there are no obverse correlations between chas and all the others.

# access the MASS package
library(MASS)
## Warning: package 'MASS' was built under R version 4.0.3
## 
## Attaching package: 'MASS'
## The following object is masked from 'package:dplyr':
## 
##     select
# load the data
data("Boston")

# center and standardize variables
boston_scaled <- scale(Boston)

# summaries of the scaled variables
summary(boston_scaled)
##       crim                 zn               indus              chas        
##  Min.   :-0.419367   Min.   :-0.48724   Min.   :-1.5563   Min.   :-0.2723  
##  1st Qu.:-0.410563   1st Qu.:-0.48724   1st Qu.:-0.8668   1st Qu.:-0.2723  
##  Median :-0.390280   Median :-0.48724   Median :-0.2109   Median :-0.2723  
##  Mean   : 0.000000   Mean   : 0.00000   Mean   : 0.0000   Mean   : 0.0000  
##  3rd Qu.: 0.007389   3rd Qu.: 0.04872   3rd Qu.: 1.0150   3rd Qu.:-0.2723  
##  Max.   : 9.924110   Max.   : 3.80047   Max.   : 2.4202   Max.   : 3.6648  
##       nox                rm               age               dis         
##  Min.   :-1.4644   Min.   :-3.8764   Min.   :-2.3331   Min.   :-1.2658  
##  1st Qu.:-0.9121   1st Qu.:-0.5681   1st Qu.:-0.8366   1st Qu.:-0.8049  
##  Median :-0.1441   Median :-0.1084   Median : 0.3171   Median :-0.2790  
##  Mean   : 0.0000   Mean   : 0.0000   Mean   : 0.0000   Mean   : 0.0000  
##  3rd Qu.: 0.5981   3rd Qu.: 0.4823   3rd Qu.: 0.9059   3rd Qu.: 0.6617  
##  Max.   : 2.7296   Max.   : 3.5515   Max.   : 1.1164   Max.   : 3.9566  
##       rad               tax             ptratio            black        
##  Min.   :-0.9819   Min.   :-1.3127   Min.   :-2.7047   Min.   :-3.9033  
##  1st Qu.:-0.6373   1st Qu.:-0.7668   1st Qu.:-0.4876   1st Qu.: 0.2049  
##  Median :-0.5225   Median :-0.4642   Median : 0.2746   Median : 0.3808  
##  Mean   : 0.0000   Mean   : 0.0000   Mean   : 0.0000   Mean   : 0.0000  
##  3rd Qu.: 1.6596   3rd Qu.: 1.5294   3rd Qu.: 0.8058   3rd Qu.: 0.4332  
##  Max.   : 1.6596   Max.   : 1.7964   Max.   : 1.6372   Max.   : 0.4406  
##      lstat              medv        
##  Min.   :-1.5296   Min.   :-1.9063  
##  1st Qu.:-0.7986   1st Qu.:-0.5989  
##  Median :-0.1811   Median :-0.1449  
##  Mean   : 0.0000   Mean   : 0.0000  
##  3rd Qu.: 0.6024   3rd Qu.: 0.2683  
##  Max.   : 3.5453   Max.   : 2.9865
# class of the boston_scaled object
class(boston_scaled)
## [1] "matrix" "array"
# change the object to data frame
boston_scaled <- as.data.frame(boston_scaled)

# summary of the scaled crime rate
summary(boston_scaled$crim)
##      Min.   1st Qu.    Median      Mean   3rd Qu.      Max. 
## -0.419367 -0.410563 -0.390280  0.000000  0.007389  9.924110
# create a quantile vector of crim and print it
bins <- quantile(boston_scaled$crim)
bins
##           0%          25%          50%          75%         100% 
## -0.419366929 -0.410563278 -0.390280295  0.007389247  9.924109610
# create a categorical variable 'crime'
crime <- cut(boston_scaled$crim, breaks = bins, include.lowest = TRUE, labels = c("low", "med_low", "med_high", "high"))

# look at the table of the new factor crime
table(crime)
## crime
##      low  med_low med_high     high 
##      127      126      126      127
# remove original crim from the dataset
boston_scaled <- dplyr::select(boston_scaled, -crim)

# add the new categorical value to scaled data
boston_scaled <- data.frame(boston_scaled, crime)

# number of rows in the Boston dataset 
n <- nrow(boston_scaled)

# choose randomly 80% of the rows
ind <- sample(n,  size = n * 0.8)

# create train set
train <- boston_scaled[ind,]

# create test set 
test <- boston_scaled[-ind,]

# save the correct classes from test data
correct_classes <- test$crime

# remove the crime variable from test data
test <- dplyr::select(test, -crime)

After scaling the dataset, all the numbers become much smaller than oringinal ones and all the mean values are zero.

# linear discriminant analysis
lda.fit <- lda(crime ~ ., data = train)

# print the lda.fit object
lda.fit
## Call:
## lda(crime ~ ., data = train)
## 
## Prior probabilities of groups:
##       low   med_low  med_high      high 
## 0.2450495 0.2450495 0.2623762 0.2475248 
## 
## Group means:
##                  zn      indus         chas        nox         rm        age
## low       1.0907684 -0.9563090 -0.113254311 -0.9146628  0.4852736 -0.9230203
## med_low  -0.1305804 -0.2970385  0.006051757 -0.5394849 -0.1322013 -0.2809074
## med_high -0.3723618  0.2176045  0.247665303  0.3969967  0.0829346  0.4331957
## high     -0.4872402  1.0171519 -0.075474056  1.0153374 -0.4139301  0.7936058
##                 dis        rad        tax      ptratio      black       lstat
## low       0.9459750 -0.6837338 -0.7188105 -0.468893811  0.3750939 -0.79988852
## med_low   0.2444996 -0.5514861 -0.4364640 -0.007921566  0.3096162 -0.14073292
## med_high -0.3912664 -0.4195557 -0.2887857 -0.298436902  0.1092699  0.02796119
## high     -0.8588370  1.6377820  1.5138081  0.780373633 -0.7672078  0.90327422
##                  medv
## low       0.585331561
## med_low   0.003681631
## med_high  0.137884388
## high     -0.664753412
## 
## Coefficients of linear discriminants:
##                 LD1         LD2         LD3
## zn       0.10923753  0.64382105 -0.84819989
## indus    0.08210700 -0.37785283 -0.03775215
## chas    -0.09941844 -0.07690272  0.07583532
## nox      0.35174779 -0.69629022 -1.38790995
## rm      -0.07532794 -0.07515575 -0.18995272
## age      0.27411026 -0.28550264 -0.19614819
## dis     -0.06046674 -0.18423840 -0.19066110
## rad      3.28684656  0.79995831 -0.25957190
## tax     -0.13011190  0.21269131  0.78859602
## ptratio  0.11494927  0.02481176 -0.16490981
## black   -0.11451867 -0.01053111  0.09270955
## lstat    0.26580816 -0.14739515  0.26694382
## medv     0.22228819 -0.23645932 -0.23146183
## 
## Proportion of trace:
##    LD1    LD2    LD3 
## 0.9433 0.0438 0.0129
# the function for lda biplot arrows
lda.arrows <- function(x, myscale = 1, arrow_heads = 0.1, color = "orange", tex = 0.75, choices = c(1,2)){
  heads <- coef(x)
  arrows(x0 = 0, y0 = 0, 
         x1 = myscale * heads[,choices[1]], 
         y1 = myscale * heads[,choices[2]], col=color, length = arrow_heads)
  text(myscale * heads[,choices], labels = row.names(heads), 
       cex = tex, col=color, pos=3)
}

# target classes as numeric
classes <- as.numeric(train$crime)

# plot the lda results
plot(lda.fit, dimen = 2, col = classes, pch = classes)
lda.arrows(lda.fit, myscale = 1)

# predict classes with test data
lda.pred <- predict(lda.fit, newdata = test)

# cross tabulate the results
table(correct = correct_classes, predicted = lda.pred$class)
##           predicted
## correct    low med_low med_high high
##   low       14      10        4    0
##   med_low    6      17        4    0
##   med_high   0       7       12    1
##   high       0       0        0   27
r = getOption("repos")
r["CRAN"] = "http://cran.us.r-project.org"
options(repos = r)

# access the MASS package
library(MASS)

data('Boston')

# center and standardize variables
boston_scaled <- scale(Boston)

# euclidean distance matrix
dist_eu <- dist(boston_scaled)

# look at the summary of the distances
summary(dist_eu)
##    Min. 1st Qu.  Median    Mean 3rd Qu.    Max. 
##  0.1343  3.4625  4.8241  4.9111  6.1863 14.3970
# manhattan distance matrix
dist_man <- dist(boston_scaled, method = 'manhattan')

# look at the summary of the distances
summary(dist_man)
##    Min. 1st Qu.  Median    Mean 3rd Qu.    Max. 
##  0.2662  8.4832 12.6090 13.5488 17.7568 48.8618
# k-means clustering
km <-kmeans(boston_scaled, centers = 3)

# plot the Boston dataset with clusters
pairs(boston_scaled, col = km$cluster)

## Bonus

# k-means clustering
km <-kmeans(boston_scaled, centers = 3)

# plot the Boston dataset with clusters
pairs(boston_scaled, col = km$cluster)

## Superbonus

model_predictors <- dplyr::select(train, -crime)

# check the dimensions
dim(model_predictors)
## [1] 404  13
dim(lda.fit$scaling)
## [1] 13  3
# matrix multiplication
matrix_product <- as.matrix(model_predictors) %*% lda.fit$scaling
matrix_product <- as.data.frame(matrix_product)

install.packages("plotly")
## Installing package into 'C:/rlibs/4.0.1'
## (as 'lib' is unspecified)
## package 'plotly' successfully unpacked and MD5 sums checked
## 
## The downloaded binary packages are in
##  C:\Users\siqizhao\AppData\Local\Temp\RtmpsvAlbN\downloaded_packages
plotly::plot_ly(x = matrix_product$LD1, y = matrix_product$LD2, z =  matrix_product$LD3, type= 'scatter3d', mode='markers', color=train$crime)
## Warning: `arrange_()` is deprecated as of dplyr 0.7.0.
## Please use `arrange()` instead.
## See vignette('programming') for more help
## This warning is displayed once every 8 hours.
## Call `lifecycle::last_warnings()` to see where this warning was generated.

Dimensionality reduction techniques

human <- read.table("http://s3.amazonaws.com/assets.datacamp.com/production/course_2218/datasets/human2.txt", sep  =",", header = T)
str(human)
## 'data.frame':    155 obs. of  8 variables:
##  $ Edu2.FM  : num  1.007 0.997 0.983 0.989 0.969 ...
##  $ Labo.FM  : num  0.891 0.819 0.825 0.884 0.829 ...
##  $ Edu.Exp  : num  17.5 20.2 15.8 18.7 17.9 16.5 18.6 16.5 15.9 19.2 ...
##  $ Life.Exp : num  81.6 82.4 83 80.2 81.6 80.9 80.9 79.1 82 81.8 ...
##  $ GNI      : int  64992 42261 56431 44025 45435 43919 39568 52947 42155 32689 ...
##  $ Mat.Mor  : int  4 6 6 5 6 7 9 28 11 8 ...
##  $ Ado.Birth: num  7.8 12.1 1.9 5.1 6.2 3.8 8.2 31 14.5 25.3 ...
##  $ Parli.F  : num  39.6 30.5 28.5 38 36.9 36.9 19.9 19.4 28.2 31.4 ...
summary(human) 
##     Edu2.FM          Labo.FM          Edu.Exp         Life.Exp    
##  Min.   :0.1717   Min.   :0.1857   Min.   : 5.40   Min.   :49.00  
##  1st Qu.:0.7264   1st Qu.:0.5984   1st Qu.:11.25   1st Qu.:66.30  
##  Median :0.9375   Median :0.7535   Median :13.50   Median :74.20  
##  Mean   :0.8529   Mean   :0.7074   Mean   :13.18   Mean   :71.65  
##  3rd Qu.:0.9968   3rd Qu.:0.8535   3rd Qu.:15.20   3rd Qu.:77.25  
##  Max.   :1.4967   Max.   :1.0380   Max.   :20.20   Max.   :83.50  
##       GNI            Mat.Mor         Ado.Birth         Parli.F     
##  Min.   :   581   Min.   :   1.0   Min.   :  0.60   Min.   : 0.00  
##  1st Qu.:  4198   1st Qu.:  11.5   1st Qu.: 12.65   1st Qu.:12.40  
##  Median : 12040   Median :  49.0   Median : 33.60   Median :19.30  
##  Mean   : 17628   Mean   : 149.1   Mean   : 47.16   Mean   :20.91  
##  3rd Qu.: 24512   3rd Qu.: 190.0   3rd Qu.: 71.95   3rd Qu.:27.95  
##  Max.   :123124   Max.   :1100.0   Max.   :204.80   Max.   :57.50

install.packages(‘FactoMineR’)

library(GGally)
library(ggplot2)
library(dplyr)
library(corrplot)
## Warning: package 'corrplot' was built under R version 4.0.3
## corrplot 0.84 loaded
library(tidyr)
library(FactoMineR)
## Warning: package 'FactoMineR' was built under R version 4.0.3
ggpairs(human)

cor(human) %>% corrplot

In this dataset, there are in total 155 observations of 8 variables.

Form the plot of pairs, we ccould see that almost all the parament are not normal distribution. As for the relationships between different variaables, there is an significantly negative relationship betweeb Mat.Mor and life.Exp, while positive corrlation between life.Exp and Edu.Exp. Seems that Parli.F doesn’t have obversly relationships with any other variables, according to the corrplot.

pca_human <- prcomp(human)
s1 <- summary(pca_human)
s1
## Importance of components:
##                              PC1      PC2   PC3   PC4   PC5   PC6    PC7    PC8
## Standard deviation     1.854e+04 185.5219 25.19 11.45 3.766 1.566 0.1912 0.1591
## Proportion of Variance 9.999e-01   0.0001  0.00  0.00 0.000 0.000 0.0000 0.0000
## Cumulative Proportion  9.999e-01   1.0000  1.00  1.00 1.000 1.000 1.0000 1.0000
biplot(pca_human, choices = 1:2, cex = c(0.8, 1), col = c("grey40", "deeppink2"))
## Warning in arrows(0, 0, y[, 1L] * 0.8, y[, 2L] * 0.8, col = col[2L], length =
## arrow.len): zero-length arrow is of indeterminate angle and so skipped

## Warning in arrows(0, 0, y[, 1L] * 0.8, y[, 2L] * 0.8, col = col[2L], length =
## arrow.len): zero-length arrow is of indeterminate angle and so skipped

## Warning in arrows(0, 0, y[, 1L] * 0.8, y[, 2L] * 0.8, col = col[2L], length =
## arrow.len): zero-length arrow is of indeterminate angle and so skipped

## Warning in arrows(0, 0, y[, 1L] * 0.8, y[, 2L] * 0.8, col = col[2L], length =
## arrow.len): zero-length arrow is of indeterminate angle and so skipped

## Warning in arrows(0, 0, y[, 1L] * 0.8, y[, 2L] * 0.8, col = col[2L], length =
## arrow.len): zero-length arrow is of indeterminate angle and so skipped

human_std <- scale(human)
summary(human_std)
##     Edu2.FM           Labo.FM           Edu.Exp           Life.Exp      
##  Min.   :-2.8189   Min.   :-2.6247   Min.   :-2.7378   Min.   :-2.7188  
##  1st Qu.:-0.5233   1st Qu.:-0.5484   1st Qu.:-0.6782   1st Qu.:-0.6425  
##  Median : 0.3503   Median : 0.2316   Median : 0.1140   Median : 0.3056  
##  Mean   : 0.0000   Mean   : 0.0000   Mean   : 0.0000   Mean   : 0.0000  
##  3rd Qu.: 0.5958   3rd Qu.: 0.7350   3rd Qu.: 0.7126   3rd Qu.: 0.6717  
##  Max.   : 2.6646   Max.   : 1.6632   Max.   : 2.4730   Max.   : 1.4218  
##       GNI             Mat.Mor          Ado.Birth          Parli.F       
##  Min.   :-0.9193   Min.   :-0.6992   Min.   :-1.1325   Min.   :-1.8203  
##  1st Qu.:-0.7243   1st Qu.:-0.6496   1st Qu.:-0.8394   1st Qu.:-0.7409  
##  Median :-0.3013   Median :-0.4726   Median :-0.3298   Median :-0.1403  
##  Mean   : 0.0000   Mean   : 0.0000   Mean   : 0.0000   Mean   : 0.0000  
##  3rd Qu.: 0.3712   3rd Qu.: 0.1932   3rd Qu.: 0.6030   3rd Qu.: 0.6127  
##  Max.   : 5.6890   Max.   : 4.4899   Max.   : 3.8344   Max.   : 3.1850
pca_human_std <- prcomp(human_std)
s2 <- summary(pca_human_std)
s2
## Importance of components:
##                           PC1    PC2     PC3     PC4     PC5     PC6     PC7
## Standard deviation     2.0708 1.1397 0.87505 0.77886 0.66196 0.53631 0.45900
## Proportion of Variance 0.5361 0.1624 0.09571 0.07583 0.05477 0.03595 0.02634
## Cumulative Proportion  0.5361 0.6984 0.79413 0.86996 0.92473 0.96069 0.98702
##                            PC8
## Standard deviation     0.32224
## Proportion of Variance 0.01298
## Cumulative Proportion  1.00000
biplot(pca_human_std, choices = 1:2, cex = c(0.8, 1), col = c("grey40", "deeppink2"))

pca_pr_std <- round(100*s2$importance[2,], digits = 1) 
pca_pr_std
##  PC1  PC2  PC3  PC4  PC5  PC6  PC7  PC8 
## 53.6 16.2  9.6  7.6  5.5  3.6  2.6  1.3
pc_lab_std <- paste0(names(pca_pr_std), " (", pca_pr_std, "%)")
biplot(pca_human_std, cex = c(0.8, 1), col = c("grey40", "deeppink2"), xlab = pc_lab_std[1], ylab = pc_lab_std[2])

If we use oringinal data to do the PCA, we can see that PC1 could explain almost 100% of variables, which means the reduction of dimensions is not succssful.After scalling them, the results look more sensible. This process tells us that scalling or standaring data before PCA is necessary, but I would guess this is especially important to data with unmormal distribution.

library(GGally)
library(corrplot)
library(magrittr)
## Warning: package 'magrittr' was built under R version 4.0.2
## 
## Attaching package: 'magrittr'
## The following object is masked from 'package:tidyr':
## 
##     extract
data(tea)
str(tea)
## 'data.frame':    300 obs. of  36 variables:
##  $ breakfast       : Factor w/ 2 levels "breakfast","Not.breakfast": 1 1 2 2 1 2 1 2 1 1 ...
##  $ tea.time        : Factor w/ 2 levels "Not.tea time",..: 1 1 2 1 1 1 2 2 2 1 ...
##  $ evening         : Factor w/ 2 levels "evening","Not.evening": 2 2 1 2 1 2 2 1 2 1 ...
##  $ lunch           : Factor w/ 2 levels "lunch","Not.lunch": 2 2 2 2 2 2 2 2 2 2 ...
##  $ dinner          : Factor w/ 2 levels "dinner","Not.dinner": 2 2 1 1 2 1 2 2 2 2 ...
##  $ always          : Factor w/ 2 levels "always","Not.always": 2 2 2 2 1 2 2 2 2 2 ...
##  $ home            : Factor w/ 2 levels "home","Not.home": 1 1 1 1 1 1 1 1 1 1 ...
##  $ work            : Factor w/ 2 levels "Not.work","work": 1 1 2 1 1 1 1 1 1 1 ...
##  $ tearoom         : Factor w/ 2 levels "Not.tearoom",..: 1 1 1 1 1 1 1 1 1 2 ...
##  $ friends         : Factor w/ 2 levels "friends","Not.friends": 2 2 1 2 2 2 1 2 2 2 ...
##  $ resto           : Factor w/ 2 levels "Not.resto","resto": 1 1 2 1 1 1 1 1 1 1 ...
##  $ pub             : Factor w/ 2 levels "Not.pub","pub": 1 1 1 1 1 1 1 1 1 1 ...
##  $ Tea             : Factor w/ 3 levels "black","Earl Grey",..: 1 1 2 2 2 2 2 1 2 1 ...
##  $ How             : Factor w/ 4 levels "alone","lemon",..: 1 3 1 1 1 1 1 3 3 1 ...
##  $ sugar           : Factor w/ 2 levels "No.sugar","sugar": 2 1 1 2 1 1 1 1 1 1 ...
##  $ how             : Factor w/ 3 levels "tea bag","tea bag+unpackaged",..: 1 1 1 1 1 1 1 1 2 2 ...
##  $ where           : Factor w/ 3 levels "chain store",..: 1 1 1 1 1 1 1 1 2 2 ...
##  $ price           : Factor w/ 6 levels "p_branded","p_cheap",..: 4 6 6 6 6 3 6 6 5 5 ...
##  $ age             : int  39 45 47 23 48 21 37 36 40 37 ...
##  $ sex             : Factor w/ 2 levels "F","M": 2 1 1 2 2 2 2 1 2 2 ...
##  $ SPC             : Factor w/ 7 levels "employee","middle",..: 2 2 4 6 1 6 5 2 5 5 ...
##  $ Sport           : Factor w/ 2 levels "Not.sportsman",..: 2 2 2 1 2 2 2 2 2 1 ...
##  $ age_Q           : Factor w/ 5 levels "15-24","25-34",..: 3 4 4 1 4 1 3 3 3 3 ...
##  $ frequency       : Factor w/ 4 levels "1/day","1 to 2/week",..: 1 1 3 1 3 1 4 2 3 3 ...
##  $ escape.exoticism: Factor w/ 2 levels "escape-exoticism",..: 2 1 2 1 1 2 2 2 2 2 ...
##  $ spirituality    : Factor w/ 2 levels "Not.spirituality",..: 1 1 1 2 2 1 1 1 1 1 ...
##  $ healthy         : Factor w/ 2 levels "healthy","Not.healthy": 1 1 1 1 2 1 1 1 2 1 ...
##  $ diuretic        : Factor w/ 2 levels "diuretic","Not.diuretic": 2 1 1 2 1 2 2 2 2 1 ...
##  $ friendliness    : Factor w/ 2 levels "friendliness",..: 2 2 1 2 1 2 2 1 2 1 ...
##  $ iron.absorption : Factor w/ 2 levels "iron absorption",..: 2 2 2 2 2 2 2 2 2 2 ...
##  $ feminine        : Factor w/ 2 levels "feminine","Not.feminine": 2 2 2 2 2 2 2 1 2 2 ...
##  $ sophisticated   : Factor w/ 2 levels "Not.sophisticated",..: 1 1 1 2 1 1 1 2 2 1 ...
##  $ slimming        : Factor w/ 2 levels "No.slimming",..: 1 1 1 1 1 1 1 1 1 1 ...
##  $ exciting        : Factor w/ 2 levels "exciting","No.exciting": 2 1 2 2 2 2 2 2 2 2 ...
##  $ relaxing        : Factor w/ 2 levels "No.relaxing",..: 1 1 2 2 2 2 2 2 2 2 ...
##  $ effect.on.health: Factor w/ 2 levels "effect on health",..: 2 2 2 2 2 2 2 2 2 2 ...
dim(tea)
## [1] 300  36
keep_columns <- c("Tea", "How", "how", "sugar", "where", "lunch")
tea_time <- dplyr::select(tea, one_of(keep_columns))
summary(tea_time)
##         Tea         How                      how           sugar    
##  black    : 74   alone:195   tea bag           :170   No.sugar:155  
##  Earl Grey:193   lemon: 33   tea bag+unpackaged: 94   sugar   :145  
##  green    : 33   milk : 63   unpackaged        : 36                 
##                  other:  9                                          
##                   where           lunch    
##  chain store         :192   lunch    : 44  
##  chain store+tea shop: 78   Not.lunch:256  
##  tea shop            : 30                  
## 
str(tea_time)
## 'data.frame':    300 obs. of  6 variables:
##  $ Tea  : Factor w/ 3 levels "black","Earl Grey",..: 1 1 2 2 2 2 2 1 2 1 ...
##  $ How  : Factor w/ 4 levels "alone","lemon",..: 1 3 1 1 1 1 1 3 3 1 ...
##  $ how  : Factor w/ 3 levels "tea bag","tea bag+unpackaged",..: 1 1 1 1 1 1 1 1 2 2 ...
##  $ sugar: Factor w/ 2 levels "No.sugar","sugar": 2 1 1 2 1 1 1 1 1 1 ...
##  $ where: Factor w/ 3 levels "chain store",..: 1 1 1 1 1 1 1 1 2 2 ...
##  $ lunch: Factor w/ 2 levels "lunch","Not.lunch": 2 2 2 2 2 2 2 2 2 2 ...
gather(tea_time) %>% ggplot(aes(value)) + facet_wrap("key", scales = "free") + geom_bar() + theme(axis.text.x = element_text(angle = 45, hjust = 1, size = 8))
## Warning: attributes are not identical across measure variables;
## they will be dropped

mca <- MCA(tea_time, graph = FALSE)
summary(mca)
## 
## Call:
## MCA(X = tea_time, graph = FALSE) 
## 
## 
## Eigenvalues
##                        Dim.1   Dim.2   Dim.3   Dim.4   Dim.5   Dim.6   Dim.7
## Variance               0.279   0.261   0.219   0.189   0.177   0.156   0.144
## % of var.             15.238  14.232  11.964  10.333   9.667   8.519   7.841
## Cumulative % of var.  15.238  29.471  41.435  51.768  61.434  69.953  77.794
##                        Dim.8   Dim.9  Dim.10  Dim.11
## Variance               0.141   0.117   0.087   0.062
## % of var.              7.705   6.392   4.724   3.385
## Cumulative % of var.  85.500  91.891  96.615 100.000
## 
## Individuals (the 10 first)
##                       Dim.1    ctr   cos2    Dim.2    ctr   cos2    Dim.3
## 1                  | -0.298  0.106  0.086 | -0.328  0.137  0.105 | -0.327
## 2                  | -0.237  0.067  0.036 | -0.136  0.024  0.012 | -0.695
## 3                  | -0.369  0.162  0.231 | -0.300  0.115  0.153 | -0.202
## 4                  | -0.530  0.335  0.460 | -0.318  0.129  0.166 |  0.211
## 5                  | -0.369  0.162  0.231 | -0.300  0.115  0.153 | -0.202
## 6                  | -0.369  0.162  0.231 | -0.300  0.115  0.153 | -0.202
## 7                  | -0.369  0.162  0.231 | -0.300  0.115  0.153 | -0.202
## 8                  | -0.237  0.067  0.036 | -0.136  0.024  0.012 | -0.695
## 9                  |  0.143  0.024  0.012 |  0.871  0.969  0.435 | -0.067
## 10                 |  0.476  0.271  0.140 |  0.687  0.604  0.291 | -0.650
##                       ctr   cos2  
## 1                   0.163  0.104 |
## 2                   0.735  0.314 |
## 3                   0.062  0.069 |
## 4                   0.068  0.073 |
## 5                   0.062  0.069 |
## 6                   0.062  0.069 |
## 7                   0.062  0.069 |
## 8                   0.735  0.314 |
## 9                   0.007  0.003 |
## 10                  0.643  0.261 |
## 
## Categories (the 10 first)
##                        Dim.1     ctr    cos2  v.test     Dim.2     ctr    cos2
## black              |   0.473   3.288   0.073   4.677 |   0.094   0.139   0.003
## Earl Grey          |  -0.264   2.680   0.126  -6.137 |   0.123   0.626   0.027
## green              |   0.486   1.547   0.029   2.952 |  -0.933   6.111   0.107
## alone              |  -0.018   0.012   0.001  -0.418 |  -0.262   2.841   0.127
## lemon              |   0.669   2.938   0.055   4.068 |   0.531   1.979   0.035
## milk               |  -0.337   1.420   0.030  -3.002 |   0.272   0.990   0.020
## other              |   0.288   0.148   0.003   0.876 |   1.820   6.347   0.102
## tea bag            |  -0.608  12.499   0.483 -12.023 |  -0.351   4.459   0.161
## tea bag+unpackaged |   0.350   2.289   0.056   4.088 |   1.024  20.968   0.478
## unpackaged         |   1.958  27.432   0.523  12.499 |  -1.015   7.898   0.141
##                     v.test     Dim.3     ctr    cos2  v.test  
## black                0.929 |  -1.081  21.888   0.382 -10.692 |
## Earl Grey            2.867 |   0.433   9.160   0.338  10.053 |
## green               -5.669 |  -0.108   0.098   0.001  -0.659 |
## alone               -6.164 |  -0.113   0.627   0.024  -2.655 |
## lemon                3.226 |   1.329  14.771   0.218   8.081 |
## milk                 2.422 |   0.013   0.003   0.000   0.116 |
## other                5.534 |  -2.524  14.526   0.197  -7.676 |
## tea bag             -6.941 |  -0.065   0.183   0.006  -1.287 |
## tea bag+unpackaged  11.956 |   0.019   0.009   0.000   0.226 |
## unpackaged          -6.482 |   0.257   0.602   0.009   1.640 |
## 
## Categorical variables (eta2)
##                      Dim.1 Dim.2 Dim.3  
## Tea                | 0.126 0.108 0.410 |
## How                | 0.076 0.190 0.394 |
## how                | 0.708 0.522 0.010 |
## sugar              | 0.065 0.001 0.336 |
## where              | 0.702 0.681 0.055 |
## lunch              | 0.000 0.064 0.111 |
plot(mca, invisible=c("ind"), habillage = "quali")

Dim1 can explain approximitalt 15.24% of variables, mostly unpackageed and tea shop with positive relationships.Dim2 can explain approximitalt 14.23% of variables, mostly other and chain store+tea shop with positive relationships.

Longitudinal analysis

library(dplyr) 
library(corrplot)
library(GGally)
library(FactoMineR)
library(factoextra)
## Warning: package 'factoextra' was built under R version 4.0.3
## Welcome! Want to learn more? See two factoextra-related books at https://goo.gl/ve3WBa
library(ggplot2)
library(tidyr)
library(MASS)
library(readr)
## Warning: package 'readr' was built under R version 4.0.2
BPRS <- read.table("https://raw.githubusercontent.com/KimmoVehkalahti/MABS/master/Examples/data/BPRS.txt", sep  =" ", header = T)

summary(BPRS)
##    treatment      subject          week0           week1           week2     
##  Min.   :1.0   Min.   : 1.00   Min.   :24.00   Min.   :23.00   Min.   :26.0  
##  1st Qu.:1.0   1st Qu.: 5.75   1st Qu.:38.00   1st Qu.:35.00   1st Qu.:32.0  
##  Median :1.5   Median :10.50   Median :46.00   Median :41.00   Median :38.0  
##  Mean   :1.5   Mean   :10.50   Mean   :48.00   Mean   :46.33   Mean   :41.7  
##  3rd Qu.:2.0   3rd Qu.:15.25   3rd Qu.:58.25   3rd Qu.:54.25   3rd Qu.:49.0  
##  Max.   :2.0   Max.   :20.00   Max.   :78.00   Max.   :95.00   Max.   :75.0  
##      week3           week4           week5           week6           week7     
##  Min.   :24.00   Min.   :20.00   Min.   :20.00   Min.   :19.00   Min.   :18.0  
##  1st Qu.:29.75   1st Qu.:28.00   1st Qu.:26.00   1st Qu.:22.75   1st Qu.:23.0  
##  Median :36.50   Median :34.50   Median :30.50   Median :28.50   Median :30.0  
##  Mean   :39.15   Mean   :36.35   Mean   :32.55   Mean   :31.23   Mean   :32.2  
##  3rd Qu.:44.50   3rd Qu.:43.00   3rd Qu.:38.00   3rd Qu.:37.00   3rd Qu.:38.0  
##  Max.   :76.00   Max.   :66.00   Max.   :64.00   Max.   :64.00   Max.   :62.0  
##      week8      
##  Min.   :20.00  
##  1st Qu.:22.75  
##  Median :28.00  
##  Mean   :31.43  
##  3rd Qu.:35.25  
##  Max.   :75.00
str(BPRS)
## 'data.frame':    40 obs. of  11 variables:
##  $ treatment: int  1 1 1 1 1 1 1 1 1 1 ...
##  $ subject  : int  1 2 3 4 5 6 7 8 9 10 ...
##  $ week0    : int  42 58 54 55 72 48 71 30 41 57 ...
##  $ week1    : int  36 68 55 77 75 43 61 36 43 51 ...
##  $ week2    : int  36 61 41 49 72 41 47 38 39 51 ...
##  $ week3    : int  43 55 38 54 65 38 30 38 35 55 ...
##  $ week4    : int  41 43 43 56 50 36 27 31 28 53 ...
##  $ week5    : int  40 34 28 50 39 29 40 26 22 43 ...
##  $ week6    : int  38 28 29 47 32 33 30 26 20 43 ...
##  $ week7    : int  47 28 25 42 38 27 31 25 23 39 ...
##  $ week8    : int  51 28 24 46 32 25 31 24 21 32 ...
BPRS$treatment <- factor(BPRS$treatment)
BPRS$subject <- factor(BPRS$subject)


BPRSL <-  BPRS %>% gather(key = weeks, value = bprs, -treatment, -subject)
BPRSL <-  BPRSL %>% mutate(week = as.integer(substr(weeks,5,5)))

# read the RATS data
RATS <- read.table("https://raw.githubusercontent.com/KimmoVehkalahti/MABS/master/Examples/data/rats.txt", header = TRUE, sep = '\t')

# Factor variables ID and Group
RATS$ID <- factor(RATS$ID)
RATS$Group <- factor(RATS$Group)

# Glimpse the data
glimpse(RATS)
## Rows: 16
## Columns: 13
## $ ID    <fct> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
## $ Group <fct> 1, 1, 1, 1, 1, 1, 1, 1, 2, 2, 2, 2, 3, 3, 3, 3
## $ WD1   <int> 240, 225, 245, 260, 255, 260, 275, 245, 410, 405, 445, 555, 4...
## $ WD8   <int> 250, 230, 250, 255, 260, 265, 275, 255, 415, 420, 445, 560, 4...
## $ WD15  <int> 255, 230, 250, 255, 255, 270, 260, 260, 425, 430, 450, 565, 4...
## $ WD22  <int> 260, 232, 255, 265, 270, 275, 270, 268, 428, 440, 452, 580, 4...
## $ WD29  <int> 262, 240, 262, 265, 270, 275, 273, 270, 438, 448, 455, 590, 4...
## $ WD36  <int> 258, 240, 265, 268, 273, 277, 274, 265, 443, 460, 455, 597, 4...
## $ WD43  <int> 266, 243, 267, 270, 274, 278, 276, 265, 442, 458, 451, 595, 4...
## $ WD44  <int> 266, 244, 267, 272, 273, 278, 271, 267, 446, 464, 450, 595, 5...
## $ WD50  <int> 265, 238, 264, 274, 276, 284, 282, 273, 456, 475, 462, 612, 5...
## $ WD57  <int> 272, 247, 268, 273, 278, 279, 281, 274, 468, 484, 466, 618, 5...
## $ WD64  <int> 278, 245, 269, 275, 280, 281, 284, 278, 478, 496, 472, 628, 5...
# Convert data to long form
RATSL <- RATS %>%
  gather(key = WD, value = Weight, -ID, -Group) %>%
  mutate(Time = as.integer(substr(WD,3,4))) 

# Glimpse the data
glimpse(RATSL)
## Rows: 176
## Columns: 5
## $ ID     <fct> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 1, 2,...
## $ Group  <fct> 1, 1, 1, 1, 1, 1, 1, 1, 2, 2, 2, 2, 3, 3, 3, 3, 1, 1, 1, 1, ...
## $ WD     <chr> "WD1", "WD1", "WD1", "WD1", "WD1", "WD1", "WD1", "WD1", "WD1...
## $ Weight <int> 240, 225, 245, 260, 255, 260, 275, 245, 410, 405, 445, 555, ...
## $ Time   <int> 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 8, 8, 8, 8, ...
names(BPRSL)
## [1] "treatment" "subject"   "weeks"     "bprs"      "week"
str(BPRSL)
## 'data.frame':    360 obs. of  5 variables:
##  $ treatment: Factor w/ 2 levels "1","2": 1 1 1 1 1 1 1 1 1 1 ...
##  $ subject  : Factor w/ 20 levels "1","2","3","4",..: 1 2 3 4 5 6 7 8 9 10 ...
##  $ weeks    : chr  "week0" "week0" "week0" "week0" ...
##  $ bprs     : int  42 58 54 55 72 48 71 30 41 57 ...
##  $ week     : int  0 0 0 0 0 0 0 0 0 0 ...
summary(BPRSL)
##  treatment    subject       weeks                bprs            week  
##  1:180     1      : 18   Length:360         Min.   :18.00   Min.   :0  
##  2:180     2      : 18   Class :character   1st Qu.:27.00   1st Qu.:2  
##            3      : 18   Mode  :character   Median :35.00   Median :4  
##            4      : 18                      Mean   :37.66   Mean   :4  
##            5      : 18                      3rd Qu.:43.00   3rd Qu.:6  
##            6      : 18                      Max.   :95.00   Max.   :8  
##            (Other):252
names(RATSL)
## [1] "ID"     "Group"  "WD"     "Weight" "Time"
str(RATSL)
## 'data.frame':    176 obs. of  5 variables:
##  $ ID    : Factor w/ 16 levels "1","2","3","4",..: 1 2 3 4 5 6 7 8 9 10 ...
##  $ Group : Factor w/ 3 levels "1","2","3": 1 1 1 1 1 1 1 1 2 2 ...
##  $ WD    : chr  "WD1" "WD1" "WD1" "WD1" ...
##  $ Weight: int  240 225 245 260 255 260 275 245 410 405 ...
##  $ Time  : int  1 1 1 1 1 1 1 1 1 1 ...
summary(RATSL)
##        ID      Group       WD                Weight           Time      
##  1      : 11   1:88   Length:176         Min.   :225.0   Min.   : 1.00  
##  2      : 11   2:44   Class :character   1st Qu.:267.0   1st Qu.:15.00  
##  3      : 11   3:44   Mode  :character   Median :344.5   Median :36.00  
##  4      : 11                             Mean   :384.5   Mean   :33.55  
##  5      : 11                             3rd Qu.:511.2   3rd Qu.:50.00  
##  6      : 11                             Max.   :628.0   Max.   :64.00  
##  (Other):110
write.csv(BPRSL, file = "BPRSL")
write.csv(RATSL, file = "RATSL")
library(ggplot2)
library(dplyr)
library(tidyr)
BPRSL <- read.csv("BPRSL", row.names = 1)
BPRSL$subject <- BPRSL$subject + 20*(BPRSL$treatment == 2)
BPRSL$treatment <- factor(BPRSL$treatment)
BPRSL$subject <- factor(BPRSL$subject)
dim(BPRSL)
## [1] 360   5
str(BPRSL)
## 'data.frame':    360 obs. of  5 variables:
##  $ treatment: Factor w/ 2 levels "1","2": 1 1 1 1 1 1 1 1 1 1 ...
##  $ subject  : Factor w/ 40 levels "1","2","3","4",..: 1 2 3 4 5 6 7 8 9 10 ...
##  $ weeks    : chr  "week0" "week0" "week0" "week0" ...
##  $ bprs     : int  42 58 54 55 72 48 71 30 41 57 ...
##  $ week     : int  0 0 0 0 0 0 0 0 0 0 ...
ggplot(BPRSL, aes(x = week, y = bprs, group = subject)) +
  geom_line() +
  facet_grid(. ~ treatment, labeller = label_both) +
  theme(legend.position = "none") + 
  scale_y_continuous(limits = c(min(BPRSL$bprs), max(BPRSL$bprs)))

The BPRS data set consists of 176 observations of 6 variables. The data records the brief psychiatric rating scale (bprs) measured from 40 male subjects divided into two treatment groups, over a period of 8 weeks.

BPRS_reg <- lm(bprs ~ week + treatment, data = BPRSL)
summary(BPRS_reg)
## 
## Call:
## lm(formula = bprs ~ week + treatment, data = BPRSL)
## 
## Residuals:
##     Min      1Q  Median      3Q     Max 
## -22.454  -8.965  -3.196   7.002  50.244 
## 
## Coefficients:
##             Estimate Std. Error t value Pr(>|t|)    
## (Intercept)  46.4539     1.3670  33.982   <2e-16 ***
## week         -2.2704     0.2524  -8.995   <2e-16 ***
## treatment2    0.5722     1.3034   0.439    0.661    
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
## 
## Residual standard error: 12.37 on 357 degrees of freedom
## Multiple R-squared:  0.1851, Adjusted R-squared:  0.1806 
## F-statistic: 40.55 on 2 and 357 DF,  p-value: < 2.2e-16
library(lme4)
## Warning: package 'lme4' was built under R version 4.0.3
## Loading required package: Matrix
## 
## Attaching package: 'Matrix'
## The following objects are masked from 'package:tidyr':
## 
##     expand, pack, unpack
BPRS_ref <- lmer(bprs ~ week + treatment + (1 | subject), data = BPRSL, REML = FALSE)
summary(BPRS_ref)
## Linear mixed model fit by maximum likelihood  ['lmerMod']
## Formula: bprs ~ week + treatment + (1 | subject)
##    Data: BPRSL
## 
##      AIC      BIC   logLik deviance df.resid 
##   2582.9   2602.3  -1286.5   2572.9      355 
## 
## Scaled residuals: 
##      Min       1Q   Median       3Q      Max 
## -2.27506 -0.59909 -0.06104  0.44226  3.15835 
## 
## Random effects:
##  Groups   Name        Variance Std.Dev.
##  subject  (Intercept) 97.39    9.869   
##  Residual             54.23    7.364   
## Number of obs: 360, groups:  subject, 40
## 
## Fixed effects:
##             Estimate Std. Error t value
## (Intercept)  46.4539     2.3521  19.750
## week         -2.2704     0.1503 -15.104
## treatment2    0.5722     3.2159   0.178
## 
## Correlation of Fixed Effects:
##            (Intr) week  
## week       -0.256       
## treatment2 -0.684  0.000
BPRS_ref1 <- lmer(bprs ~ week + treatment + (week | subject), data = BPRSL, REML = FALSE)
summary(BPRS_ref1)
## Linear mixed model fit by maximum likelihood  ['lmerMod']
## Formula: bprs ~ week + treatment + (week | subject)
##    Data: BPRSL
## 
##      AIC      BIC   logLik deviance df.resid 
##   2523.2   2550.4  -1254.6   2509.2      353 
## 
## Scaled residuals: 
##     Min      1Q  Median      3Q     Max 
## -2.4655 -0.5150 -0.0920  0.4347  3.7353 
## 
## Random effects:
##  Groups   Name        Variance Std.Dev. Corr 
##  subject  (Intercept) 167.827  12.955        
##           week          2.331   1.527   -0.67
##  Residual              36.747   6.062        
## Number of obs: 360, groups:  subject, 40
## 
## Fixed effects:
##             Estimate Std. Error t value
## (Intercept)  45.9830     2.6470  17.372
## week         -2.2704     0.2713  -8.370
## treatment2    1.5139     3.1392   0.482
## 
## Correlation of Fixed Effects:
##            (Intr) week  
## week       -0.545       
## treatment2 -0.593  0.000
anova(BPRS_ref1, BPRS_ref)
## Data: BPRSL
## Models:
## BPRS_ref: bprs ~ week + treatment + (1 | subject)
## BPRS_ref1: bprs ~ week + treatment + (week | subject)
##           npar    AIC    BIC  logLik deviance  Chisq Df Pr(>Chisq)    
## BPRS_ref     5 2582.9 2602.3 -1286.5   2572.9                         
## BPRS_ref1    7 2523.2 2550.4 -1254.6   2509.2 63.663  2  1.499e-14 ***
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
BPRS_ref2 <- lmer(bprs ~ week * treatment + (week | subject), data = BPRSL, REML = FALSE)
summary(BPRS_ref2)
## Linear mixed model fit by maximum likelihood  ['lmerMod']
## Formula: bprs ~ week * treatment + (week | subject)
##    Data: BPRSL
## 
##      AIC      BIC   logLik deviance df.resid 
##   2523.5   2554.5  -1253.7   2507.5      352 
## 
## Scaled residuals: 
##     Min      1Q  Median      3Q     Max 
## -2.4747 -0.5256 -0.0866  0.4435  3.7884 
## 
## Random effects:
##  Groups   Name        Variance Std.Dev. Corr 
##  subject  (Intercept) 164.204  12.814        
##           week          2.203   1.484   -0.66
##  Residual              36.748   6.062        
## Number of obs: 360, groups:  subject, 40
## 
## Fixed effects:
##                 Estimate Std. Error t value
## (Intercept)      47.8856     2.9840  16.047
## week             -2.6283     0.3752  -7.006
## treatment2       -2.2911     4.2200  -0.543
## week:treatment2   0.7158     0.5306   1.349
## 
## Correlation of Fixed Effects:
##             (Intr) week   trtmn2
## week        -0.668              
## treatment2  -0.707  0.473       
## wek:trtmnt2  0.473 -0.707 -0.668
anova(BPRS_ref2, BPRS_ref1)
## Data: BPRSL
## Models:
## BPRS_ref1: bprs ~ week + treatment + (week | subject)
## BPRS_ref2: bprs ~ week * treatment + (week | subject)
##           npar    AIC    BIC  logLik deviance Chisq Df Pr(>Chisq)
## BPRS_ref1    7 2523.2 2550.4 -1254.6   2509.2                    
## BPRS_ref2    8 2523.5 2554.6 -1253.7   2507.5  1.78  1     0.1821
Fitted <- fitted(BPRS_ref2)
BPRSL <- BPRSL %>%
  mutate(Fitted)
ggplot(BPRSL, aes(x = week, y = Fitted, linetype = subject)) +
  geom_line() +
  scale_linetype_manual(values = rep(1:10, times=4)) +
  facet_grid(. ~ treatment, labeller = label_both) +
  theme(legend.position = "none") + 
  scale_y_continuous(limits = c(min(BPRSL$Fitted), max(BPRSL$Fitted)))

RATSL <- read.csv("RATSL", row.names = 1)
RATSL$ID <- factor(RATSL$ID)
RATSL$Group <- factor(RATSL$Group)
dim(RATSL)
## [1] 176   5
str(RATSL)
## 'data.frame':    176 obs. of  5 variables:
##  $ ID    : Factor w/ 16 levels "1","2","3","4",..: 1 2 3 4 5 6 7 8 9 10 ...
##  $ Group : Factor w/ 3 levels "1","2","3": 1 1 1 1 1 1 1 1 2 2 ...
##  $ WD    : chr  "WD1" "WD1" "WD1" "WD1" ...
##  $ Weight: int  240 225 245 260 255 260 275 245 410 405 ...
##  $ Time  : int  1 1 1 1 1 1 1 1 1 1 ...
library(dplyr)
library(tidyr)
library(ggplot2)
ggplot(RATSL, aes(x = Time, y = Weight, group = ID)) +
  geom_line() +
  facet_grid(. ~ Group, labeller = label_both) +
  scale_y_continuous(limits = c(min(RATSL$Weight), max(RATSL$Weight)))

RATSL <- RATSL %>%
  group_by(Time) %>%
  mutate(stdWeight = (Weight - mean(Weight))/sd(Weight)) %>%
  ungroup()
ggplot(RATSL, aes(x = Time, y = stdWeight, group = ID)) +
  geom_line() +
  facet_grid(. ~ Group, labeller = label_both) +
  scale_y_continuous(name = "standardized Weight")

n <- RATSL$Time %>% unique() %>% length()
RATSS <- RATSL %>%
  group_by(Group, Time) %>%
  summarise(mean = mean(Weight), se = sd(Weight)/sqrt(n)) %>%
  ungroup()
## `summarise()` regrouping output by 'Group' (override with `.groups` argument)
ggplot(RATSS, aes(x = Time, y = mean, linetype = Group, shape = Group)) +
  geom_line() +
  scale_linetype_manual(values = c(1,2,3)) +
  geom_point(size=3) +
  scale_shape_manual(values = c(1,2,3)) +
  geom_errorbar(aes(ymin = mean - se, ymax = mean + se, linetype="1"), width=0.3) +
  theme(legend.position = c(0.9,0.5)) +
  scale_y_continuous(name = "mean(Weight) +/- se(Weight)")

RATSL8S <- RATSL %>%
  filter(Time > 1) %>%
  group_by(Group, ID) %>%
  summarise(mean = mean(Weight)) %>%
  ungroup()
## `summarise()` regrouping output by 'Group' (override with `.groups` argument)
ggplot(RATSL8S, aes(x = Group, y = mean)) +
  geom_boxplot() +
  stat_summary(fun.y = "mean", geom = "point", shape=23, size=4, fill = "white") +
  scale_y_continuous(name = "mean(Weight), weeks 2-9")
## Warning: `fun.y` is deprecated. Use `fun` instead.

RATSL8S1 <- RATSL8S %>%
  filter(mean > 250 & mean < 550 & (mean < 475 | mean > 525))
ggplot(RATSL8S1, aes(x = Group, y = mean)) +
  geom_boxplot() +
  stat_summary(fun.y = "mean", geom = "point", shape=23, size=4, fill = "white") +
  scale_y_continuous(name = "mean(Weight), weeks 2-9")
## Warning: `fun.y` is deprecated. Use `fun` instead.

RATS <- read.table("https://raw.githubusercontent.com/KimmoVehkalahti/MABS/master/Examples/data/rats.txt", sep="", header=TRUE)
RATSL8S2 <- RATSL8S %>%
  mutate(baseline = RATS$WD1)
fit <- lm(mean ~ baseline + Group, data = RATSL8S2)
summary(fit)
## 
## Call:
## lm(formula = mean ~ baseline + Group, data = RATSL8S2)
## 
## Residuals:
##     Min      1Q  Median      3Q     Max 
## -23.905  -4.194   2.190   7.577  14.800 
## 
## Coefficients:
##             Estimate Std. Error t value Pr(>|t|)    
## (Intercept) 33.16375   21.87657   1.516   0.1554    
## baseline     0.92513    0.08572  10.793 1.56e-07 ***
## Group2      34.85753   18.82308   1.852   0.0888 .  
## Group3      23.67526   23.25324   1.018   0.3287    
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
## 
## Residual standard error: 11.68 on 12 degrees of freedom
## Multiple R-squared:  0.9936, Adjusted R-squared:  0.992 
## F-statistic: 622.1 on 3 and 12 DF,  p-value: 1.989e-13
anova(fit)
## Analysis of Variance Table
## 
## Response: mean
##           Df Sum Sq Mean Sq   F value   Pr(>F)    
## baseline   1 253625  253625 1859.8201 1.57e-14 ***
## Group      2    879     439    3.2219  0.07586 .  
## Residuals 12   1636     136                       
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

```